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KMID : 0379120180460020186
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Korean Journal of Mycology
2018 Volume.46 No. 2 p.186 ~ p.192
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Molecular Markers for the Rapid Detection of Colletotrichum coccodes, an Anthracnose Pathogen of Tomato
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Kim Jun-Young
Jang Si-Woon
Kim Hyun-Ju
Kim Seong-Hwan
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Abstract
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Rapid and accurate detection methods for Colletotrichum coccodes, an anthracnose pathogen of pepper and tomato, were developed using PCR. A specific primer set, coccoTef-F/coccoTef-R, which was constructed by analyzing tef-1α genes from 13 species and 22 strains of Colletotrichum, could specifically detect C. coccodes at a level of 10 ng by conventional PCR method and at 10 pg by real-time PCR. The PCR-based methods were also capable of detecting C. coccodes in pepper and tomato seeds artificially infected with the pathogen. The developed PCR methods can be applied for rapid and accurate inspection of C. coccodes in the seeds intended for export or import.
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KEYWORD
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Anthracnose, Colletotrichum coccodes, Molecular marker
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